How to Use a TCI Machine for Dog Breeding

By thetopdogpetstore | Published: 2026-10-22

Category: How-to Guides

Learn the step-by-step process of using a TCI machine for canine transcervical insemination, including setup, procedure, and common pitfalls.

Quick answer

A TCI machine enables transcervical insemination in dogs without surgery. Follow the steps below to perform the procedure safely and effectively.

Why Use a TCI Machine?

Transcervical insemination (TCI) avoids the need for surgical insemination, reducing stress and recovery time for the bitch. The TCI machine uses a specialized catheter to navigate the cervix, allowing semen to be deposited directly into the uterus.

This method is particularly useful when natural mating is not possible or when using chilled or frozen semen. It requires practice to master, but offers a valuable tool for breeders.

Step-by-Step TCI Procedure

Prepare the TCI Machine and Supplies: Assemble the TCI machine according to the manufacturer's instructions. Have a sterile, lubricated catheter ready. Warm the semen to body temperature (approx. 98-100°F) if using chilled or frozen. Common mistake: using a cold catheter or semen can cause uterine contractions and reduce success.
Position and Restrain the Bitch: Place the bitch in a standing position on a non-slip surface. Have an assistant hold her steady. Do not sedate unless necessary, as sedation can affect cervical relaxation. Checkpoint: Ensure the bitch is calm and comfortable to minimize movement during the procedure.
Insert the Catheter and Navigate the Cervix: Gently insert the lubricated catheter into the vagina. Use the TCI machine's light source to visualize the cervix. Advance the catheter through the cervical os with a gentle twisting motion. Common mistake: forcing the catheter can cause trauma. If resistance is felt, reposition and try again.
Deposit the Semen: Once the catheter is through the cervix, slowly depress the syringe to deposit the semen into the uterus. Avoid rapid injection, which can cause backflow. Checkpoint: Observe for any semen reflux; if it occurs, the catheter may not be properly placed.
Post-Insemination Care: Keep the bitch calm and upright for 10-15 minutes to help retain the semen. Clean the TCI machine and catheter according to the manufacturer's guidelines. Common mistake: allowing the bitch to lie down immediately can cause semen leakage.

Product Facts

TCI machine: The TCI machine is offered by thetopdogpetstore as a single Standard option. This listing provides the basic unit; be sure to check included components or purchase additional catheters separately.
TCI machine